In vivo murine model of continuous intramedullary infusion of particles—A preliminary study

✍ Scribed by Ting Ma; Steven G. Ortiz; Zhinong Huang; Peigen Ren; R. Lane Smith; Stuart B. Goodman

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 181 KB
• Volume: 88B
• Category: Article
• ISSN: 1552-4973
• DOI: 10.1002/jbm.b.31175

✦ Synopsis

Abstract

Continued production of wear debris affects both initial osseointegration and subsequent bone remodeling of total joint replacements (TJRs). However, continuous delivery of clinically relevant particles using a viable, cost effective, quantitative animal model to simulate the scenario in humans has been a challenge for orthopedic researchers. In this study, we successfully infused blue‐dyed polystyrene particles, similar in size to wear debris in humans, to the intramedullary space of the mouse femur for 4 weeks using an osmotic pump. Approximately 40% of the original particle load (85 μL) was delivered into the intramedullary space, an estimate of 3 × 10^9^ particles. The visible blue dye carried by the particles confirmed the delivery. This model demonstrated that continuous infusion of particles to the murine bone‐implant interface is possible. In vivo biological processes associated using wear debris particles can be studied using this new animal model. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009