## Abstract Certain experimental manipulations with sea urchin oocytes, eggs, and early embryos result in induction of cell death. We were interested in whether or not these cells posessed functional apoptotic machinery, and whether cellular demise under certain experimental conditions is due to ac
In vivo mitochondrial DNA-protein interactions in sea urchin eggs and embryos
โ Scribed by Marina Roberti; Paola Loguercio Polosa; Clara Musicco; Francesco Milella; Sohail A. Qureshi; Maria N. Gadaleta; Howard T. Jacobs; P. Cantatore
- Publisher
- Springer-Verlag
- Year
- 1999
- Tongue
- English
- Weight
- 353 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0172-8083
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Adenosine 3',5'-cyclic monophosphate (CAMP) binds t o high-molecular substances which are probably proteins, in homogenates of sea urchin eggs and embryos. The bound CAMP is exchangeable. Optimal pH for the binding capacity of the proteins with CAMP is 4.0, and is shifted t o 5.0 in the presence of
## Abstract Contents of the tricarboxylic acid (TCA) cycle intermediates, coenzyme A (CoA) derivatives, and carnitine derivatives were estimated in the developing eggs and embryos of the sea urchin, __Anthocidaris crassispina__. Levels of the TCA cycle intermediates in unfertilized eggs were simila
## Abstract Phosphatase activities in sea urchin eggs and plutei were investigated by means of histochemical staining of immunoprecipitates. Two protein fractions were obtained by extraction in a hypotonic medium and by detergent treatment of the residual pellet. Three distinctly different phosphat