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Adenosine 3?,5?-cyclic monophosphate-binding protein in sea urchin eggs and embryos

✍ Scribed by Fujino, Yukio ;Yasumasu, Ikuo

Publisher: Wiley (John Wiley & Sons)
Year: 1978
Weight: 390 KB
Volume: 1
Category: Article
ISSN: 0148-7280
DOI: 10.1002/mrd.1120010207

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✦ Synopsis

Adenosine 3',5'-cyclic monophosphate (CAMP) binds t o high-molecular substances which are probably proteins, in homogenates of sea urchin eggs and embryos. The bound CAMP is exchangeable. Optimal pH for the binding capacity of the proteins with CAMP is 4.0, and is shifted t o 5.0 in the presence of 5 mM caffeine. The level of bound cAMP increases steeply during 10 minutes of incubation. This is then followed by a less steep increase. The level of bound CAMP decreases in the presence of NaC1. The dissociation constant between cAMP and the proteins in homogenates of unfertilized and fertilized eggs is about 10 nM, and the value in the embryos at the gastrula stage is lower than that in the unfertilized egg homogenate.

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