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In vivo decline of methotrexate and methotrexate polyglutamates in age-fractionated erythrocytes

✍ Scribed by Henrik Schrøder; Karsten Fogh; Troels Herlin


Publisher
Springer
Year
1988
Tongue
English
Weight
650 KB
Volume
21
Category
Article
ISSN
0344-5704

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A rapid, quantitative procedure for separating methotrexate (MTX) polyglutamates utilizing molecular-sieve column chromatography has been developed. MTX polyglutamates were separated in 25 min and detected by a radioassay with a sensitivity of 0.1 pmol MTX, allowing analysis on 2 to 5 mg of tissue.