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In vivo and in vitro models of ionizing radiation to the vocal folds

✍ Scribed by Benjamin Saltman; Dennis H. Kraus; Hazel Szeto; Bhupesh Parashar; Ronald Ghossein; Diane Felsen; Ryan C. Branski

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
363 KB
Volume
32
Category
Article
ISSN
1043-3074

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Background

Radiation therapy (RT) to the head and neck often results in damage to the vocal folds (VF) and surrounding structures. Characterization and treatment of these sequelae is limited, likely due to the lack of experimental models.

Methods

Larynges from rats exposed to 2 fractionation schedules (40 Gy total) were analyzed histologically. In vitro, reactive oxygen species (ROS) synthesis, and transcription of select genes associated with ROS, inflammation, and fibrosis were examined in VF fibroblasts after single‐dose radiation.

Results

Although radiation‐induced histologic alterations are made to VF architecture, 1 fractionation schedule was accompanied by significant morbidity and mortality. In vitro, radiation increased ROS synthesis and inflammatory and profibrotic gene expression.

Conclusion

Our data suggest that hyperfractionated RT is more tolerable. Utilizing this model, RT‐induced histologic aberrations are made to the VF mucosa. In addition, a relationship between radiation, ROS, and inflammatory and fibrotic gene expression was observed in vitro. Β© 2009 Wiley Periodicals, Inc. Head Neck, 2010

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