Nature of mutation in the human hprt gene following in vivo exposure to ionizing radiation of cesium-137

✍ Scribed by A. D. da Cruz; B. W. Glickman

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 94 KB
• Volume: 30
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/(sici)1098-2280(1997)30:4<385::aid-em3>3.0.co;2-i

✦ Synopsis

The current study comprises the analysis of muta-assay with genomic DNA. Missense mutations were tions in 10 individuals accidentally exposed to ce-the most frequent event recovered, comprising 40% sium-137 during the 1987 radiological accident in (23/57) of the spectral sample. An excess of events Goia ˆnia, Brazil. Their exposures were among the involving A:T base pairs was observed, exhibiting highest experienced, ranging from 1 to 7 Gy. Pe-a significant difference (x 2 Å 12.7, P Å 0.0004) ripheral T-lymphocyte samples were obtained 3.3 when compared to the spontaneous spectrum. This years after the original exposure and mutation was finding may reflect the effect of ionizing radiationstudied at the hprt locus using the 6-thioguanine-induced damage, suggesting a potential similarity resistance selection assay. The mutational spectrum to radiation effects in prokaryotes. At the genomic for the exposed population is comprised of 90 inde-level, 36.7% (33/90) of the mutants exhibited gross pendent mutants. Based on T-cell receptor analysis, structural alterations, as detected by multiplex PCR. only 5% (5/95) were clonally related. Mutants were Deletion events were over-represented in our specinitially studied using RT-PCR and directly se-tral sample, displaying a twofold increase when quenced using an automated laser fluorescent DNA compared to the frequency observed in the spontasequencer. Mutants that repeatedly failed to pro-neous mutation database.