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In vivo 13C saturation transfer effect of the lactate dehydrogenase reaction

✍ Scribed by Su Xu; Jehoon Yang; Jun Shen

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
708 KB
Volume
57
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

Lactate dehydrogenase (LDH, EC 1.1.1.27) catalyzes an exchange reaction between pyruvate and lactate. It is demonstrated here that this reaction is sufficiently fast to cause a significant magnetization (saturation) transfer effect when the ^13^C resonance of pyruvate is saturated by a continuous‐wave (CW) RF pulse. Infusion of [2‐^13^C]glucose was used to allow labeling of pyruvate C2 at 207.9 ppm to determine the pseudo first‐order rate constant of the unidirectional lactate → pyruvate flux in vivo. During systemic administration of GABA~A~ receptor antagonist bicuculline, this pseudo first‐order rate constant was determined to be 0.08 ± 0.01 s^−1^ (mean ± SD, N = 4) in halothane‐anesthetized adult rat brains. In 9L and C6 rat glioma models, the ^13^C saturation transfer effect of the LDH reaction was also detected in vivo. Our results demonstrate that the ^13^C magnetization transfer effect of the LDH reaction may be useful as a novel marker for utilizing noninvasive in vivo MRS to study many physiological and pathological conditions, such as cancer. Magn Reson Med 57:258–264, 2007. © 2007 Wiley‐Liss, Inc.

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