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In vitro31P NMR spectroscopy of the reproductive organs of the male rat: An improved preparation method

✍ Scribed by J. Der Van Grond; D. Seijkens; W. P. Th. M. Mali

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
352 KB
Volume
15
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

In vitro ^31^P spectroscopy can be applied to disentangle the broad resonances in in vivo ^31^P NMR spectra of the reproductive organs of the male rat. However, adenosinetriphosphate (ATP) and glycerophosphocholine (GPC), present in large amounts in vivo, are nearly completely absent in vitro. With a modified PCA extraction method, in which the organs were lyophilized before extraction, a large amount of ATP and GPC could be found in vitro. This new extraction method is probably not only restricted to in vitro analysis of the testis but is likely to be useful for analysis of other organs such as kidney and liver. Β© 1990 Academic Press, Inc.

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