In vitro selective effect of melphalan on human T-cell populations

✍ Scribed by Shlomo Ben-Efraim; Luise Komlos; Jaffa Notmann; Jacob Hart; Isaac Halbrecht

• Publisher: Springer-Verlag
• Year: 1985
• Tongue: English
• Weight: 428 KB
• Volume: 19
• Category: Article
• ISSN: 0340-7004
• DOI: 10.1007/bf00199312

✦ Synopsis

In vitro treatment with 2 ~tg/2 x 106 cells melphalan (L-PAM: L-phenylalanine mustard) significantly decreased the total number of T lymphocytes from peripheral blood (PBL) of healthy human donors and of the OKT 4 population (precursor suppressor~helper~inducer) T cells as defined by monoclonal antibodies OKT3 and OKT4, respectively. No changes in the OKT~8 lymphocyte population (cytotoxic/mature suppressor cells) were observed following the same treatment. Preincubation of PBL with L-PAM at concentrations that do not affect the rate of DNA synthesis in PHA-stimulated lymphocytes inhibited the generation of T suppressor lymphocytes by ConA, as shown by their effect on PHA stimulation. Treatment of allogeneic PBL with L-PAM had no effect on mature suppressor T cells already induced by Con A, as shown by incubation of PBL with L-PAM after incubation with ConA.