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In vitro regression of cement gland epithelium fromXenopus laevis

✍ Scribed by Lyerla, Timothy A.

Publisher: John Wiley and Sons
Year: 1975
Tongue: English
Weight: 302 KB
Volume: 193
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1401930317

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✦ Synopsis

Abstract

The development of Xenopus laevis cement gland ectoderm cultured as epidermal vesicles from neurula stage embryos is described. The life cycle explanted gland cells closely parallels that seen in intact animals. The cells increase in height, extrude their secretory mucin and most of the oocyte pigment granule inclusions, and then regress. The entire sequence takes from 16 to 21 days at 18°C. The vesicles show no obvious changes in general appearance after gland cell senescence and remain viable up to 35 days of culture. Cement gland development progresses normally then in the absence of any influences that might come from the embryo and appears to be an autonomous sequence of the gland cells once they begin to differentiate in early neurula stages.

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