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Acid phosphatase activity in the development of the cement gland inXenopus laevis

✍ Scribed by Ling, Brenda Yu-Feng ;Lyerla, Timothy A.

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
588 KB
Volume
195
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

The properties of acid phosphatase from the cement gland of Xenopus laevis embryos were investigated both biochemically and electrophoretically. The studies were made on excised glands at a preregressing stage (stage 35/36, Nieuwkoop and Faber staging series) and a regressing stage (stage 45). The total activity (amount of p‐nitrophenol released/hr/gland) of acid phosphatase increased 1.7‐fold while the specific activity (amount of p‐nitrophenol released/hr/μg protein) increased 2.8‐fold from stage 35/36 to stage 45. There was some indication of a changing pH pattern during regression. Acid phosphatase activity showed a slight peak at pH 4.5 at stage 35/36 but a sharp optimal peak at pH 5.0 at stage 45. Also, there was increased phosphatase activity in both isotonic and hypotonic homogenates, in the absence of Triton X‐100, during gland cell regression. Electrophoretic separation of acid phosphatases from cement gland homogenates resolved two isozymes at stage 35/36, and two more forms appeared at stage 45. Both the quantitative and qualitative changes seen during cement gland development suggest a correlation between acid phosphatase activity and gland cell regression.

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