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In vitro packaging of covalently closed circular monomers of bacteriophage DNA

โœ Scribed by Gail J. Pruss; James C. Wang; Richard Calendar


Book ID
119443375
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
909 KB
Volume
98
Category
Article
ISSN
0022-2836

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Quantitative binding of covalently close
โœ Joel Bresser; David Gillespie ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 696 KB

A procedure for quantitativel y binding covalently closed circular DNA to nitrocellulose is detailed. The procedure involves making DNA saturated with respect to NaI at 25ยฐC, denaturing the DNA by heating to 80-100 cC, then passing the DNA-NaI solution through a nitrocellulose membrane at 50-60ยฐC. F