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Quantitative binding of covalently closed circular DNA to nitrocellulose in NaI

✍ Scribed by Joel Bresser; David Gillespie

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
696 KB
Volume
129
Category
Article
ISSN
0003-2697

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✦ Synopsis

A procedure for quantitativel y binding covalently closed circular DNA to nitrocellulose is detailed. The procedure involves making DNA saturated with respect to NaI at 25Β°C, denaturing the DNA by heating to 80-100 cC, then passing the DNA-NaI solution through a nitrocellulose membrane at 50-60Β°C. Form I DNA remains denatured throughout the loading procedure and is quantitatively deposited on the membrane as denatured DNA. The mechanism of binding DNA to nitrocellulose in Nal appears to be different from the binding prom oted by NaCl.

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