In Vitro Mutagenesis Protocols
β Michael K. Trower
π Library
π
1996
π Humana Press
π English
β¦ LIBER β¦
π
In Vitro Mutagenesis Protocols: Third Edition
β Scribed by Miguel Alcalde (auth.), Jeff Braman (eds.)
- Publisher
- Humana Press
- Year
- 2010
- Tongue
- English
- Leaves
- 432
- Series
- Methods in Molecular Biology 634
- Edition
- 3
- Category
- Library
β¬ Acquire This Volume
No coin nor oath required. For personal study only.
β¦ Synopsis
In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biologyβ’ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.
β¦ Table of Contents
Front Matter....Pages i-xiv
Front Matter....Pages 1-1
Front Matter....Pages 3-14
Front Matter....Pages 15-35
Front Matter....Pages 37-52
Front Matter....Pages 53-74
Front Matter....Pages 75-86
Front Matter....Pages 87-100
Front Matter....Pages 102-102
Back Matter....Pages 103-109
....Pages 111-126
β¦ Subjects
Human Genetics; Proteomics
π SIMILAR VOLUMES
In Vitro Mutagenesis Protocols
β Priya Handa, Swapna Thanedar, Umesh Varshney (auth.), Jeff Braman (eds.)
π Library
π
2002
π Humana Press
π English
In Vitro Mutagenesis Protocols
β Priya Handa, Swapna Thanedar, Umesh Varshney (auth.), Jeff Braman (eds.)
π Library
π
2002
π Humana Press
π English
Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications
In Vitro Mutagenesis Protocols
β Michael K. Trower
π Library
π
1996
π Humana Press
π English
In In Vitro Mutagenesis Protocols leading experts from industrial and academic laboratories describe easily reproducible procedures for site-directed and random mutagenesis. Site-directed protocols include those based on strand-selection, PCR (including "splicing by overlap extension" and the "megap
In Vitro Mutagenesis Protocols
β Priya Handa, Swapna Thanedar, Umesh Varshney (auth.), Jeff Braman (eds.)
π Library
π
2002
π Humana
π English
Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications
In Vitro Mutagenesis: Methods and Protoc
β Andrew Reeves
π Library
π
2016
π Humana Press
π English
<p><i>In vitro</i> mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performingΒ <i>in vitro</i>Β mutagenesis using such methods as genome editing, transposon (