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In Vitro Mutagenesis Protocols

✍ Scribed by Priya Handa, Swapna Thanedar, Umesh Varshney (auth.), Jeff Braman (eds.)


Publisher
Humana
Year
2002
Tongue
English
Leaves
305
Edition
2nd ed
Category
Library

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✦ Synopsis


Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today's molecular biologists reliable and powerful techniques with which to illuminate the proteome.

✦ Table of Contents


Front Matter....Pages i-xvi
Back Matter....Pages 1-6
....Pages 7-17

✦ Subjects


Cell Biology


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