In Vitro Mutagenesis Protocols
β Michael K. Trower
π Library
π
1996
π Humana Press
π English
β¦ LIBER β¦
π
In Vitro Mutagenesis Protocols
β Scribed by Priya Handa, Swapna Thanedar, Umesh Varshney (auth.), Jeff Braman (eds.)
- Publisher
- Humana Press
- Year
- 2002
- Tongue
- English
- Leaves
- 305
- Series
- Methods in Molecular Biology 182
- Edition
- 2nd ed
- Category
- Library
β¬ Acquire This Volume
No coin nor oath required. For personal study only.
β¦ Synopsis
Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today's molecular biologists reliable and powerful techniques with which to illuminate the proteome.
β¦ Table of Contents
Front Matter....Pages i-xvi
Back Matter....Pages 1-6
....Pages 7-17
β¦ Subjects
Cell Biology
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