In-vitro-Maturation
✍ Scribed by S. von Otte; B. Schöpper; S. Al Hasani; R. Felberbaum; K. Diedrich
- Publisher
- Springer
- Year
- 2004
- Tongue
- German
- Weight
- 377 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0017-5994
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Oocytes isolated from mature follicles undergo spontaneous maturation when cultured in vitro. Forskolin, an adenylate cyclase stimulator, inhibited resumption of meiosis of cumulus-free mouse oocytes in vitro. Germinal vesicle breakdown (GVBD) was prevented in more than 85% of the oocytes treated by
## Abstract The in vitro culture of fully grown mammalian oocytes results in spontaneous meiotic maturation from prophase arrest to metaphase II. This maturation can be inhibited by steroid hormones in both murine and porcine oocytes. Using selected steroids, we have examined the structure‐activity