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Forskolin and mouse oocyte maturation in vitro

✍ Scribed by Sato, Eimei ;Koide, S. S.

Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
541 KB
Volume
230
Category
Article
ISSN
0022-104X

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✦ Synopsis

Oocytes isolated from mature follicles undergo spontaneous maturation when cultured in vitro. Forskolin, an adenylate cyclase stimulator, inhibited resumption of meiosis of cumulus-free mouse oocytes in vitro. Germinal vesicle breakdown (GVBD) was prevented in more than 85% of the oocytes treated by forskolin at concentrations of 20 micrograms/ml and higher. The inhibiting effect of forskolin was dose-dependent and reversible. FSH, LH, FSH plus LH, estrogen, progesterone, and estrogen plus progesterone did not reverse the block induced by forskolin in cumulus-free and cumulus-enclosed oocytes. The present results suggest that intracellular cAMP may play a role in the regulation of oocyte maturation.

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