Phosphoglucomutase, the enzyme which catalyses the intcrconversion of glucose-l-phosphate and glucose-6-phosphate, was crystallized from r a b b i t skeletal muscle b y โขAJJAIr (1948) and b y JAGANNATHAN and LUCK (1949) and obtained in a highly purified form from yeast b y ~. AJJAI~ and McCoY (1958)
In vitro lipase synthesis in aspergillus niger
โ Scribed by C. V. Ramakrishnan
- Publisher
- Springer
- Year
- 1957
- Tongue
- English
- Weight
- 153 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0028-1042
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Sorbitol dehydrogenase has been purified about 26 fold from a strain of Aspergillus niger, growing on sorbitol as the sole source of carbon. An absolute specificity of this enzyme for sorbitol, fructose, NAD and NADtt was observed. The Km for sorbitol and fructose were found to be 9.8 X 10 -5 M and
A strain of Aspergillus niger adapted to grow on gluconate is cultivated on a medium containing gluconic acid lactone as the sole carbon source. Nutritional requirements of the adapted mold were studied. The organism shows optimum growth at 30 ~ C in a medium containing per litre 20 g of gluconic ac