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In vitro investigations of β-agonist accumulation in the eye

✍ Scribed by Inge Dürsch; Heinrich H.D. Meyer; Stefan Jäger

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
423 KB
Volume
275
Category
Article
ISSN
0003-2670

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✦ Synopsis

The eye IS scud to be. the most accumulatmg Ussue for &-adrenergx agonists m bovmes To avold ammal expenments, a perfusion system was developed for the eye Therefore the eyes were perfused vlil artena ophtahmca wth defined amounts of Rmger solution contammg 1 ng ml-' of the respectwe j?-agomst [clenbuterol, mabuterol, 2-ammo-3-chloro-5_(l'-hydroxy-2'-ammo-terf-butyletbylylethyl)Wrrdme (ACP), salbutamol and terbutahne] /3&omsts were extracted, cleaned-up mth ctidges contammg LChrospher 60 RP select-B and determmed by enzyme nnmunoassay The tests prove accumulation for other #I-agomsts also besldes clenbukrol and the accumulation shows good correspondence to the metabohc stab&y m the organism [clenbuterol > mabuterol > 2-ammo-3-chloro-5-(l'-hydroxy-2'-ammtie+butyl-ethyl) pyndme > salbutamol > terbutalme] The accumulation m retma + uvea was about 35 tunes higher than m vitreous humour + aqueous humour The amount of accumulated clenbuterol corresponds urlth the volume of perfused Rmger solution contammg the fi-agomst Prebmmary data mvestlgatmg the wsual system suggest a very weak, d any, mteracUon of @-agomsts Hntb opsm

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