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In vitro propagation ofLilium testaceumand structural investigation of the storage β-1,4-glucomannan

✍ Scribed by T. Wozniewski; W. Blaschek; G. Franz

Publisher: Springer
Year: 1991
Tongue: English
Weight: 342 KB
Volume: 10
Category: Article
ISSN: 0721-7714
DOI: 10.1007/bf00233814

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✦ Synopsis

Bulblet and callus cultures of Lilium testaceum were initiated in vitro from bulbscales. Continous propagation of the bulblet cultures was achieved on a modified Murashige and Skoog agar medium containing 1-naphthalene acetic acid (0.1 mg/l) and kinetin (0.1 mg/l) as phytohormones. The in vitro grown bulbs synthesized large quantities of storage ß-1,4-glucomannans (mannose: glucose = 7∶3; molecular weight = 200 kd) with an identical structure to the glucomannans from the in vivo grown bulbs. Higher 1-naphthalene acetic acid concentrations (1 mg/l) resulted in increased callus formation. Liquid suspension cultures derived from callus exhibited only small amounts of reserve glucomannans.

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