In SituDetection of Diamine Oxidase Activity Using Enhanced Chemiluminescence
β Scribed by L. Bruun; G. Houen
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 230 KB
- Volume
- 233
- Category
- Article
- ISSN
- 0003-2697
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A flow-mjectlon assay for mlututlon of chamme oxldase (DA01 IS described Hlstamme in decomposed fish IS detected and used as the enzyme substrate Sample extracts are doubly injected, one of the two resultmg sample zones IS passed through a reactor containing DA0 lmmobdlzed to Sepharose I-hstatnme IS
The activity of diamine oxidase (DAO) in amniotic fluid was measured by three different methods. The first two, one calorimetric and the other fluorometric, depended on the coupled peroxidase assay. The other was a radiochemical procedure. The latter proved to be a reliable method, free from interfe