Modified high-performance liquid chromatographic determination of diamine oxidase activity in plasma

✍ Scribed by Pier Antonio Biondi; Fabrizio Ceciliani; Cinzia Gandini; Claudio Lucarelli

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 313 KB
• Volume: 507
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)84211-4

✦ Synopsis

A previous high-performance liquid chromatographic determination of diamine oxidase activity suitable for tissue homogenates was modified in order to adapt it to plasma samples. Simple additional steps were introduced after the enzyme reaction and before the chromatographic separation, both according to the previous method. In this way the sensitivity and the reproducibility of the overall procedure was suitable for routine plasma diamine oxidase estimations.