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Flow-injection assay of enzyme inhibition in fish using immobilized diamine oxidase

✍ Scribed by James M. Hungerford; Aleksei A. Arefyev

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
961 KB
Volume
261
Category
Article
ISSN
0003-2670

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✦ Synopsis

A flow-mjectlon assay for mlututlon of chamme oxldase (DA01 IS described Hlstamme in decomposed fish IS detected and used as the enzyme substrate Sample extracts are doubly injected, one of the two resultmg sample zones IS passed through a reactor containing DA0 lmmobdlzed to Sepharose I-hstatnme IS detected sequentially m both the prlstme and enzymatically treated zones The detection chemistry (kmetlcs-optimlzed condensation with phthahc rhcarboxaldehyde) allows the selective detectIon of lustamme Subsequent cabbratlon with tustamme standard and comparison of the two peak heights allows generic detection of compounds mlututmg the DAO-medlated cunverslon of tustanune Prehnunary data show that the system can detect mlubltors m extracts of decomposed Malu-Mahi (associated with a Scombrcud poisoning outbreak) and that far lower levels of miututron are observed m fresh fish Several known mlubitors and competitive substrates of DA0 are also tested

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