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Flow-injection analysis for l-glutamate using immobilized l-glutamate oxidase: comparison of an enzyme reactor and enzyme electrode

✍ Scribed by Toshio Yao; Naokazu Kobayashi; Tamotsu Wasa

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 326 KB
Volume: 231
Category: Article
ISSN: 0003-2670
DOI: 10.1016/s0003-2670(00)86405-3

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✦ Synopsis

An enzyme electrode and enzyme reactor based on lmmoblhzed L-glutamate oxldase are used for the determmatlon of L-glutamate m a flow-mJectlon system The hydrogen peroxlde produced IS monitored amperometncally The enzyme reactor system surpasses the enzyme electrode system with regard to sensltlvlty and analytlcal speed For both systems, the peak current 1s linearly related to the L-glutamate concentration m the range 5 x 10m6-1 x 10m3 M L-Glutamate m seasomng can be determmed very selectively with < 0 7% r s.d.

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