Determination of l-glutamate by amperometric flow-injection analysis using immobilized glutamate oxidase: manifold for simultaneous detection of component signal and blank signal

✍ Scribed by Kiyoshi Matsumoto; Koji Sakoda; Yutaka Osajima

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 388 KB
• Volume: 261
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)80186-b

✦ Synopsis

L-Glutamate oxldase was unmoblhzed on Ammo-Cellulofine and used as an enzyme reactor III a flow-mjectlon system The hydrogen peroxrde produced was momtored amperometncally A new configurabon IS described for the detenmnatlon of L-glutamate m food samples for which the matnx provides varymg blank values The peak current was linearly related to L-glutamate concentration m the range 0 01-O 3 mM, and the relatnre standard devlatlon was 1 7% m ten successwe assays at the 0 3 mM level The proposed method was used for seasomng analysis and was compared with an L-glutamate lut (enzymahc, spectrophotometrs) method