𝔖 Bobbio Scriptorium
✦   LIBER   ✦

In situdetection of a DNA-polymerase activity in the nuclei of mouse spermatozoa

✍ Scribed by Ph. Chevaillier; M. Philippe

Publisher
Springer
Year
1976
Tongue
English
Weight
816 KB
Volume
54
Category
Article
ISSN
0009-5915

No coin nor oath required. For personal study only.

✦ Synopsis

A nuclear DNA-polymerase activity has been detected in situ in mouse spermatozoa with a cytochemical method. The acid-insoluble radioactive product obtained after incubation in a mixture containing all four deoxyribonucleoside 5'-triphosphates, Mg++,KCl and dithiothreitol (DTT) was completely removed by DNAse but was insensitive to RNAse and pronase action. The polymerization reaction did not take place in the presence of only one nucleotide and was dramatically reduced when actinomycin D was present; it did not seem to be inhibited markedly by N-ethylmaleimide (NEM) or by parahydroxymercuribenzoate (PHMB) but was sensitive to high concentrations of KCl.--The reaction depended strictly on the presence of nuclear DNA as template since treatment with pancreatic DNAae before the DNA-polymerase assay completely prevented the appearance of any radioactivity.

πŸ“œ SIMILAR VOLUMES

DNA polymerase activity in preimplantati
DNA polymerase activity in preimplantation mouse embryos
✍ Kiessling, A. A. ;Weitlauf, H. M. πŸ“‚ Article πŸ“… 1979 πŸ› John Wiley and Sons 🌐 English βš– 588 KB

## Abstract DNA polymerase activity was measured in mouse embryos at stages before implantation to determine whether it increases in proportion to the amount of DNA synthesis, as it does in populations of differentiated mammalian cells, or remains constant, as it does in early sea urchin embryos. T

Poly (A)Β·oligo (dT)-stimulated DNA polym
Poly (A)Β·oligo (dT)-stimulated DNA polymerase activity in preimplantation mouse embryos
✍ Kiessling, Ann A. ;Weitlauf, Harry M. πŸ“‚ Article πŸ“… 1981 πŸ› John Wiley and Sons 🌐 English βš– 362 KB

## Abstract DNA polymerase activity with Poly (A)Β·oligo(dT) as a template‐primer has been found in preimplantation mouse embryos. A sharp increase in enzyme activity was observed at the time of formation of inner cell mass (ICM) and trophoblast cells. Differential inhibition of enzyme activity befo

Release of DNA template restrictions in
Release of DNA template restrictions in rabbit spermatozoa and rat liver nuclei
✍ Zirkin, Barry R. ;Boison, Adele ;Heston, W. D. W. ;Coffey, Donald S. πŸ“‚ Article πŸ“… 1976 πŸ› John Wiley and Sons 🌐 English βš– 272 KB

## Abstract The effects of disulfide reduction on DNA template activity of rabbit sperm and rat liver nuclei are compared. After treatment of rabbit spermatozoa with 5 mM dithiothreitol and 1% Triton X‐100 for 90 minutes at room temperature, sperm nuclei decondense and nuclear template restrictions