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Poly (A)·oligo (dT)-stimulated DNA polymerase activity in preimplantation mouse embryos

✍ Scribed by Kiessling, Ann A. ;Weitlauf, Harry M.

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
362 KB
Volume
215
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

DNA polymerase activity with Poly (A)·oligo(dT) as a template‐primer has been found in preimplantation mouse embryos. A sharp increase in enzyme activity was observed at the time of formation of inner cell mass (ICM) and trophoblast cells. Differential inhibition of enzyme activity before and after ICM formation raised the possibility that more than one Poly(A)·oligo(dT)‐directed DNA polymerase was detected.

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## Abstract Cell division stops in mouse blastocysts during the prolonged, free‐living phase associated with delayed implantation. It is not known how the embryos are rendered mitotically dormant and then reactivated to implant at a later time, and the present experiments were undertaken to determi