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Release of DNA template restrictions in rabbit spermatozoa and rat liver nuclei

✍ Scribed by Zirkin, Barry R. ;Boison, Adele ;Heston, W. D. W. ;Coffey, Donald S.

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
272 KB
Volume
197
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

The effects of disulfide reduction on DNA template activity of rabbit sperm and rat liver nuclei are compared. After treatment of rabbit spermatozoa with 5 mM dithiothreitol and 1% Triton X‐100 for 90 minutes at room temperature, sperm nuclei decondense and nuclear template restrictions for DNA synthesis are released. By contrast, similar thiol treatment of isolated rat liver nuclei fails to release DNA template restrictions. These results suggest a significant difference in the control of template activity in mammalian sperm and somatic cell nuclei.

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The covalent binding of reactive metabolites of the carcinogen N-hydroxy-2-acetylaminofluorene to DNA and protein in isolated, intact rat liver nuclei was studied. The chemically synthesized 2-acetylaminofluorene-N-sulfate became covalently bound to DNA and protein to form adducts, 50% to 60% of whi