In Situ One-Step Method for Synthesis of “Click”-Functionalized Monolithic Stationary Phase for Capillary Electrochromatography

✍ Scribed by Aleksander Salwiński; Vincent Roy; Luigi A. Agrofoglio; Raphaël Delépée

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 714 KB
• Volume: 212
• Category: Article
• ISSN: 1022-1352
• DOI: 10.1002/macp.201100526

✦ Synopsis

Abstract

Propargyl acrylate‐based functionalizable monolithic stationary phase (FMSP) for capillary electrochromatography, easily modifiable by alkyne–azide 1,3‐dipolar cycloaddition, is developed. Optimization of FMSP is focused on (i) physical properties: rigidity, stability, and pore size and (ii) the ease of capillary preparation and grafting. The effect of grafting is demonstrated on the separation of eleven polycyclic aromatic hydrocarbons (PAHs), three flavonols, and chlorpheniramine before and after derivatization. Two azides are synthesized: the first, cinnamyl azide increases the retention of PAHs and improves separation of flavonols, the second, 6‐azido‐6‐deoxy‐β‐cyclodextrin allows the chiral separation of warfarin, improves the efficiency of PAHs separation and increases retention of chlorpheniramine.