One problem of matrix-assisted laser desorption ionization coupled to time-of-flight mass spectrometry is the moderate mass accuracy that typically can be obtained in routine applications, Here we report improved mass accuracy for peptides, even when low amounts and complex peptide mixtures are used
โฆ LIBER โฆ
Improving Sample Treatment for In-Solution Protein Identification by Peptide Mass Fingerprint Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
โ Scribed by Santos, H. M.; Rial-Otero, R.; Fernandes, L.; Vale, G.; Rivas, M. G.; Moura, I.; Capelo, J. L.
- Book ID
- 127259418
- Publisher
- American Chemical Society
- Year
- 2007
- Tongue
- English
- Weight
- 204 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1535-3893
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Identification of proteins using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) peptide mass fingerprinting (PMF) is a key technique in proteomics. The method is known to be sensitive as well as amenable to high-throughput operation, but the resulting identifications suffer f