Improvements in polyacrylamide gel slicing
β Scribed by Roy E. Gingery
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 395 KB
- Volume
- 78
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
An improved polyacrylamide gel slicer has been devised that provides rapid uniform slicing with a precision of 4-6%. The advantages of this type of slicer are: The gel is sliced directly from the electrophoresis tube; gel diameter and length can vary with no modification of the system; and gels with a range of acrylamide concentrations can be fractionated with no pretreatment of the gel. r Cooperative investigations,
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A method for electrophoretic elution of proteins from polyacrylamide gel slices is described. Eluted proteins were retained by a discontinuous conductivity gradient (M. Otto and M. SnejdΓ‘rkovΓ‘, Anal. Biochem. 111, 111-114 (1981)). The method has been adapted to slices from slab gels and gels that ha
A simple, economical method for counting acrylamide gel slices on solid filter paper supports in a toluene-based scintillation cocktail is described. Major advantages of the system include no requirement for either dissolution of the gel or elution of the radioactive material prior to emulsion count