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Determination of radioactivity in polyacrylamide gel slices mounted on solid supports

✍ Scribed by John G. Leinen; James L. Wittliff

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
528 KB
Volume
86
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple, economical method for counting acrylamide gel slices on solid filter paper supports in a toluene-based scintillation cocktail is described. Major advantages of the system include no requirement for either dissolution of the gel or elution of the radioactive material prior to emulsion counting and the direct reutilization of scintillation cocktail and vials. Additionally, 3ZP-labeled RNA samples can be counted with better relative efficiencies and those labeled with 14C or 33P can be determined at equivalent efficiencies. Tritium was detected less readily, with an absolute efficiency of approximately 10%.

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A method has been developed for determination of the specific radioactivity of t-leucine in different proteins or protein subunits which have been separated by polyacrylamide gel electrophoresis. After electrophoresis, the proteins are visualized by staining with Coomassie Brilliant Blue R, and the