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Improvement of production rate on recombinant CHO cells in two-stage culture

โœ Scribed by Matsuoka, Hiroshi; Shimizu, Chie; Tazawa, Mihoko


Book ID
121591505
Publisher
BioMed Central
Year
2013
Tongue
English
Weight
300 KB
Volume
7
Category
Article
ISSN
1753-6561

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## Abstract To investigate the effect of culture temperature on erythropoietin (EPO) production and glycosylation in recombinant Chinese hamster ovary (CHO) cells, we cultivated CHO cells using a perfusion bioreactor. Cells were cultivated at 37ยฐC until viable cell concentration reached 1โ€‰ร—โ€‰10^7^ c