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Improvement in the expression efficiency of mammalian cytochrome P450 and NADPH-cytochrome P450 oxidoreductase in Saccharomyces cerevisiae

✍ Scribed by Satoru Ohgiya; Tsunahiro Kii; Takako Goda; Tamotsu Hoshino; Kan Hamabuchi; Hiroshi Yokota; Akira Yuasa; Kozo Ishizaki


Book ID
110369203
Publisher
Springer Netherlands
Year
1997
Tongue
English
Weight
272 KB
Volume
19
Category
Article
ISSN
0141-5492

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An initial survey of 18 haploid strains of Saccharomyces cerevisiae revealed that only 3 of these strains could produce a detectable level of cytochrome P-450. A cross between a cytochrome P-450 producing strain of S. cerevisiae (B/B) and a non-producing strain (D22) gave a diploid which was a non-p