๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Improvement in the antibody productivity of human-human hybridomas by transfection with Tac gene

โœ Scribed by Keiji Iwamoto; Yasushi Shintani; Reiko Sasada; Tasuku Honjo; Kazuaki Kitano

Publisher
Springer
Year
1990
Tongue
English
Weight
823 KB
Volume
4
Category
Article
ISSN
0920-9069

No coin nor oath required. For personal study only.

โœฆ Synopsis

The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5-2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor o0 antibody. To enhance the response of the hybridoma cells to rIL-2, Tac gene was introduced by co-transfection with Tac gene expression plasmid pTB459 and G418 resistant gene expression plasmid pRSVneo. Tac cDNA transfected hybridoma was induced to produce 6 times as much IgG by rIL-2 as was the control. This antibody production promoting phenomenon mediated by rIL-2 was depressed by anti-Tac antibody.

๐Ÿ“œ SIMILAR VOLUMES

Improvement in the proliferative activit
Improvement in the proliferative activity of human-human hybridomas at low cell density by transfection with bFGF gene
โœ Keiji Iwamoto; Yasushi Shintani; Hidekazu Sawada; Kazuaki Kitano ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 689 KB

Highly purified recombinant basic fibroblast growth factor (rbFGF) and acidic FGF (aFGF) stimulated the proliferation of human-human (h-h) hybridomas to the extent of over four-fold from a low cell density such as 1 x 10(3) cells per ml in a serum-free medium in 24-well plates. The stimulatory effec

Stimulation of monoclonal antibody produ
Stimulation of monoclonal antibody production by human-human hybridoma cells with an elevated concentration of potassium or sodium phosphate in serum-free medium
โœ S. Sato; H. Murakami; T. Sugahara; T. Ikegami; K. Yamada; H. Omura; S. Hashizume ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› Springer ๐ŸŒ English โš– 325 KB

Potassium or sodium phosphate was found to stimulate the production of human monoclonal antibody by human-human hybridoma HB4C5. The addition of 15 mM Na-phosphate (pH 7.4) into serum-free culture medium increased the antibody production up to 4-fold, when seeded at cell density of 1 x 10 5 cells/ml