Improved TLC systems for rapid resolution of phenyl thiohydantoin amino acids

✍ Scribed by R. Bhushan; V. K. Mahesh; P. V. Mallikharjun

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 243 KB
• Volume: 3
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1130030111

✦ Synopsis

Two new solvent systems, n-hexane + propionic acid (26: 5, v/v) and chloroform + acetone (29 : 3, v/v), for the rapid resolution and identification of an 18-component mixture of phenylthiohydantoin amino acids are reported. Using these systems certain difficult combinations of phenylthiohydantoin amino acids are resolved. Two more solvent systems, viz chloroform + acetic acid (27 : 3, v/v) and chloroform + methanol (30 : 4, v/v), are developed to resolve phenylthiodantoin derivatives of aspartic and glutamic acids. Detection of PTH-amino acids. After development, the chromatograms were removed from the chambers. These were dried and exposed to iodine vapours to locate the PTH-amino acid as light yellow-brown spots on the chromatograms.