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Improved techniques for the extraction and chromatography of cobalamins

✍ Scribed by Wayne A. Fenton; Leon E. Rosenberg

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
427 KB
Volume
90
Category
Article
ISSN
0003-2697

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✦ Synopsis

We describe the use of column chromatography on the nonpolar adsorbent, Amberlite XAD-2, and on silanized silica gel in the desalting and partial purification of cobalamins. These techniques are both simpler and more versatile than phenol extraction, without sacrificing efficiency. In addition, a solvent system for thin-layer chromatography on shanked silica gel is described which rapidly separates naturally occurring cobalamins.

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