Sonication and liquid chromatography as a rapid technique for extraction and fractionation of plant material

✍ Scribed by Silvia R. Sargenti; Walter Vichnewski

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 156 KB
• Volume: 11
• Category: Article
• ISSN: 0958-0344
• DOI: 10.1002/(sici)1099-1565(200003/04)11:2<69::aid-pca499>3.0.co;2-y

✦ Synopsis

A fast method of extraction and isolation of the major compounds of

Lychnophora ericoides Mart. is described which uses only 100 g of dried, ground plant material and small amounts of solvent and solid phases. The method combines extraction by sonication with a mixture of non-miscible solvents, rapid preseparation of the crude extract by vacuum-liquid chromatography (silica gel 60H), and separation by preparative, reversed-phase HPLC or classical liquid chromatography with polyvinylpyrrolidone. Eighteen compounds (flavonoids and sesquiterpene lactones) were isolated by this method and identified by spectrometric methods, namely 7,4'-dihydroxy-flavonol, 5,7-dihydroxy-3-methoxy-flavonol, galangine, 7,4'dihydroxy-dihydroflavonol,5,7,4'-trihydroxy-dihydroflavonol,7-hydroxy-4'-methoxy-dihydroflavonol,pinobanksin, 5,7'-dihydroxy-4'-hydroxy-flavanone, 7-hydroxy-4'-methoxy-flavanone, 5,7-dihydroxy-flavone, acacetin, 7-hydroxy-3',4'-dihydroxy-isoflavone, 15-desoxigoiazensolide, 2',3'-dihydro-15-desoxygoyazensolide, eremantholides A and C, 4,5-dihydroeremantholide A and lychnopholide.