Improved rapid sampling for in vivo kinetics of intracellular metabolites in Saccharomyces cerevisiae
β Scribed by H. C. Lange; M. Eman; G. van Zuijlen; D. Visser; J. C. van Dam; J. Frank; M. J. Teixeira de Mattos; J. J. Heijnen
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 318 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract The properties of intracellular RNase in disintegrated cell suspensions of __Saccharomyces cerevisiae__ have been studied. The influence of salt addition and/or incubation of the suspension on the activity of RNase and on the degradation of endogenous RNA was determined. No significant
## Abstract In this paper, three sampling techniques for rapid quenching of cellular metabolism and subsequent separation of cells from fermentation broth are compared: (i) quick freezing of fermentation broth directly in liquid nitrogen; (ii) quenching metabolism by exposing the fermentation broth