Critical evaluation of sampling techniques for residual glucose determination in carbon-limited chemostat culture of Saccharomyces cerevisiae
✍ Scribed by M. R. Mashego; W. M. van Gulik; J. L. Vinke; J. J. Heijnen
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 89 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
In this paper, three sampling techniques for rapid quenching of cellular metabolism and subsequent separation of cells from fermentation broth are compared: (i) quick freezing of fermentation broth directly in liquid nitrogen; (ii) quenching metabolism by exposing the fermentation broth to stainless steel beads (4‐mm diameter) in a filter syringe precooled to −18°C; and (iii) withdrawal of the filtrate through a 0.45‐μm filter attached to a syringe and a needle inserted directly into the fermentor. It was concluded that use of liquid nitrogen as a quenching method to rapidly arrest cellular metabolism, for quantitative analysis of extracellular glucose, is not a very reliable method and that the filter syringe steel beads work very well. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 395–399, 2003.