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Analysis of in vivo kinetics of glycolysis in aerobic Saccharomyces cerevisiae by application of glucose and ethanol pulses

✍ Scribed by Diana Visser; Gertan A. van Zuylen; Jan C. van Dam; Michael R. Eman; Angela Pröll; Cor Ras; Liang Wu; Walter M. van Gulik; Joseph J. Heijnen


Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
690 KB
Volume
88
Category
Article
ISSN
0006-3592

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In vivo analysis of metabolic dynamics i
✍ Uwe Theobald; Werner Mailinger; Michael Baltes; Manfred Rizzi; Matthias Reuss 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 468 KB 👁 2 views

The goal of this work was to obtain rapid sampling technique to measure transient metabolites in vivo. First, a pulse of glucose was added to a culture of the yeast Saccharomyces cerevisiae growing aerobically under glucose limitation. Next, samples were removed at 2 to 5 s intervals and quenched us

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✍ Manfred Rizzi; Michael Baltes; Uwe Theobald; Matthias Reuss 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 331 KB 👁 2 views

A mathematical model of glycolysis in Saccharomyces cerevisiae is presented. The model is based on rate equations for the individual reactions and aims to predict changes in the levels of intra-and extracellular metabolites after a glucose pulse, as described in part I of this study. Kinetic analysi