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Improved purification and properties of glucose dehydrogenase fromBacillus subtilis

✍ Scribed by Karmali, Amin; Serralheiro, Luisa

Book ID
121428090
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
997 KB
Volume
70
Category
Article
ISSN
0300-9084

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Extracellular mannanase from Bacillus subtilis NM-39, an isolate from Philippine soil, was purified about 240-fold with a yield of 7.3% by ammonium sulphate fractionation, DEAE-Toyopearl chromatography and Sephacryl S-200 gel filtration. Its Mr was 38 kDa and it had a pI of 4.8 and optimum activity