Purification and properties of mannanase
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N. S. Mendoza; M. Arai; T. Kawaguchi; T. Yoshida; L. M. Joson
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Article
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1994
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Springer
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English
โ 334 KB
Extracellular mannanase from Bacillus subtilis NM-39, an isolate from Philippine soil, was purified about 240-fold with a yield of 7.3% by ammonium sulphate fractionation, DEAE-Toyopearl chromatography and Sephacryl S-200 gel filtration. Its Mr was 38 kDa and it had a pI of 4.8 and optimum activity