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Expression, purification, and biochemical properties of arginase fromBacillus subtilis168

โœ Scribed by Yu, Jin-Ju; Park, Ki-Bum; Kim, Su-Gon; Oh, Suk-Heung


Book ID
120343407
Publisher
The Microbiological Society of Korea
Year
2013
Tongue
English
Weight
249 KB
Volume
51
Category
Article
ISSN
1225-8873

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โœ N. S. Mendoza; M. Arai; T. Kawaguchi; T. Yoshida; L. M. Joson ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Springer ๐ŸŒ English โš– 334 KB

Extracellular mannanase from Bacillus subtilis NM-39, an isolate from Philippine soil, was purified about 240-fold with a yield of 7.3% by ammonium sulphate fractionation, DEAE-Toyopearl chromatography and Sephacryl S-200 gel filtration. Its Mr was 38 kDa and it had a pI of 4.8 and optimum activity