Extracellular mannanase from Bacillus subtilis NM-39, an isolate from Philippine soil, was purified about 240-fold with a yield of 7.3% by ammonium sulphate fractionation, DEAE-Toyopearl chromatography and Sephacryl S-200 gel filtration. Its Mr was 38 kDa and it had a pI of 4.8 and optimum activity
Purification and properties of ap-nitrobenzyl esterase fromBacillus subtilis
β Scribed by Y-R Chen; S Usui; S W Queener; C-A Yu
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 847 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1476-5535
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