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Purification and properties of β-amylase fromBacillus circulansS31

✍ Scribed by H. S. Kwan; K. H. So; K. Y. Chan; S. C. Cheng

Publisher
Springer
Year
1994
Tongue
English
Weight
159 KB
Volume
10
Category
Article
ISSN
1573-0972

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✦ Synopsis

A thermotolerant //-amylase was purified from Bacillus circulans $31 isolated from soil in Hong Kong. The purified enzyme has an Mr of 64 kDa and was stable at 50°C and pH 7.0 for 30 min. Its Km for starch was 0.9 mg/ml with a Vmax of 0.3 mg/min. It was not activated by any metal ion although sulphydrys reagents were inhibitory.

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