A common problem, when using Silane A 174 to bind polyacrylamide gels to supporting glass plates, is that the gel also binds to the cover glass in the gel casting frame. This can be avoided by using a low concentration of Silane A 174 and by rinsing the supporting glass plate with acetone after the
✦ LIBER ✦
Improved procedure for silanization of glass plates as supports for polyacrylamide gels
✍ Scribed by Prof. Dr. Volker Neuhoff
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 106 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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We have developed a methodology based on experimental design, to optimize a polyacrylamide gel as the support for enzyme immobilization, taking advantage of all the properties which this type of gel has. Monomer and crosslinking agent proportions are responsible for both the porous structure and por