Silanization of supporting glass plates avoiding fixation of polyacrylamide gels to glass cover plates
✍ Scribed by HÅKan Tegelström; Per-Ivan Wyöni
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 140 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
✦ Synopsis
A common problem, when using Silane A 174 to bind polyacrylamide gels to supporting glass plates, is that the gel also binds to the cover glass in the gel casting frame. This can be avoided by using a low concentration of Silane A 174 and by rinsing the supporting glass plate with acetone after the Silane A 174 treatment.
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