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Silanization of supporting glass plates avoiding fixation of polyacrylamide gels to glass cover plates

✍ Scribed by HÅKan Tegelström; Per-Ivan Wyöni


Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
140 KB
Volume
7
Category
Article
ISSN
0173-0835

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✦ Synopsis


A common problem, when using Silane A 174 to bind polyacrylamide gels to supporting glass plates, is that the gel also binds to the cover glass in the gel casting frame. This can be avoided by using a low concentration of Silane A 174 and by rinsing the supporting glass plate with acetone after the Silane A 174 treatment.


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