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Improved plant regeneration from shed microspore culture in barley (Hordeum vulgareL.) cv. igri

✍ Scribed by A. Ziauddin; E. Simion; K. J. Kasha

Publisher: Springer
Year: 1990
Tongue: English
Weight: 876 KB
Volume: 9
Category: Article
ISSN: 0721-7714
DOI: 10.1007/bf00231551

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✦ Synopsis

This report describes rapid regeneration of green plants from microspores of the barley cultivar Igri. Use of 0.3 M mannitol during maceration and isolation was essential for response from mechanically isolated microspores of barley cv. Igri grown under our conditions. A shed microspore culture system proved to be simple and gave a fast response; plants were obtained as early as 25 days after the material was taken from the donor plant. A 28-day cold-pretreatment of spikes can also be replaced with a 3-4 day pretreatment of anthers in mannitol. Shed microspores from 100 anthers produced an average of 292 plants with 91% of them green. Approximately 80% of the regenerated plants were spontaneously doubled-haploids.

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