Improved plant regeneration from wheat anther and barley microspore culture using phenylacetic acid (PAA)
✍ Scribed by A. Ziauddin; A. Marsolais; E. Simion; K. J. Kasha
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 976 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0721-7714
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✦ Synopsis
The effect of the auxin phenylacetic acid (PAA) on wheat anther and on barley anther/microspore culture was investigated. With PAA the induction response was not usually significantly different from controls but a significantly higher number of green plants were produced in wheat anther and barley microspore culture. For wheat anther culture 100 mg/L PAA was beneficial. For barley microspore culture the optimum levels were from 1 to 100 mg/L, depending on genotype. In barley anther culture there were no improvements using PAA. In wheat anther culture, 145 green plants/100 anthers were obtained with cultivar Veery'S', while the average response from twelve F1 hybrids in the breeding program was 332 green plants/100 anthers. At least 1000 green plants were obtained using isolated microspores from 100 anthers in barley cv. Igri. With cv. Bruce, regeneration occurred only when 100 mg/L PAA was used. The influence of PAA appears at the embryogenic phase of the culture system. The possible mechanisms by which PAA may improve regeneration are discussed.